Media Summary: Brief overview of the different levels of physical organization of the genome, from the linear DNA sequence to A/B compartments. Basic overview of DNA sequencing and its applications Overview of the types of networks we will discuss in class (regulatory, protein-protein, genetic and co-expression).
Mcb 182 Lecture 10 1 - Detailed Analysis & Overview
Brief overview of the different levels of physical organization of the genome, from the linear DNA sequence to A/B compartments. Basic overview of DNA sequencing and its applications Overview of the types of networks we will discuss in class (regulatory, protein-protein, genetic and co-expression). Basic analysis goals of scRNA-seq, including clustering, trajectory inference and gene network inference. Basic experimental design considerations of scRNA-seq, including dropout noise, batch effects A deeper look at how population structure is detected, visualized, and corrected for in GWAS.
Considerations about how to choose between 3C/4C/Hi-C, and also a brief discussion of how Hi-C can be used for genome ... A brief introduction to a few non-coding RNA types, including miRNA, lncRNA and eRNA. A discussion of the role of modules (groups of highly connected genes, with few connections to other genes) in gene networks, ... Discussion of TADs and A/B compartments, as well as how to identify them from heatmap visualizations. We introduce the concept of sequence alignments to compare how similar they are.
